Clathrin light chain diversity regulates membrane deformation in vitro and synaptic vesicle formation in vivo.

ABSTRACT: Clathrin light chain (CLC) subunits in vertebrates are encoded by paralogous genes CLTA and CLTB, and both gene products are alternatively spliced in neurons. To understand how this CLC diversity influences neuronal clathrin function, we characterized the biophysical properties of clathrin comprising individual CLC variants for correlation with neuronal phenotypes of mice lacking either CLC-encoding gene. CLC splice variants differentially influenced clathrin knee conformation within assemblies, and clathrin with neuronal CLC mixtures was more effective in membrane deformation than clathrin with single neuronal isoforms nCLCa or nCLCb. Correspondingly, electrophysiological recordings revealed that neurons from mice lacking nCLCa or nCLCb were both defective in synaptic vesicl

SUBMITTER: Redlingshofer L 

PROVIDER: S-EPMC7519287 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature