Molecular insights into the genome dynamics and interactions between core and acquired genomes of <i>Vibrio cholerae</i>.

ABSTRACT: Bacterial species are hosts to horizontally acquired mobile genetic elements (MGEs), which encode virulence, toxin, antimicrobial resistance, and other metabolic functions. The bipartite genome of Vibrio cholerae harbors sporadic and conserved MGEs that contribute in the disease development and survival of the pathogens. For a comprehensive understanding of dynamics of MGEs in the bacterial genome, we engineered the genome of V. cholerae and examined in vitro and in vivo stability of genomic islands (GIs), integrative conjugative elements (ICEs), and prophages. Recombinant vectors carrying the integration module of these GIs, ICE and CTXΦ, helped us to understand the efficiency of integrations of MGEs in the V. cholerae chromosome. We have deleted more than 250 acquire

SUBMITTER: Pant A 

PROVIDER: S-EPMC7519391 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature