GDF11 enhances therapeutic efficacy of mesenchymal stem cells for myocardial infarction via YME1L-mediated OPA1 processing.

ABSTRACT: Growth differentiation factor 11 (GDF11) has been shown to promote stem cell activity, but little is known about the effect of GDF11 on viability and therapeutic efficacy of cardiac mesenchymal stem cells (MSCs) for cardiac injury. To understand the roles of GDF11 in MSCs, mouse heart-derived MSCs were transduced with lentiviral vector carrying genes for both GDF11 and green fluorescent protein (GFP) (MSCs^LV-GDF11 ) or cultured with recombinant GDF11 (MSCs^rGDF11 ). Either MSCs^rGDF11 or MSCs ^LV-GDF11 displayed less cell apoptosis and better paracrine function, as well as preserved mitochondrial morphology and function under hypoxic condition as compared with control MSCs. GDF11 enhanced phosphorylation of Smad2/3, which upregulated expression of Y

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC7519765 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature