Decellularized skeletal muscles display neurotrophic effects in three-dimensional organotypic cultures.

ABSTRACT: Skeletal muscle decellularization allows the generation of natural scaffolds that retain the extracellular matrix (ECM) mechanical integrity, biological activity, and three-dimensional (3D) architecture of the native tissue. Recent reports showed that in vivo implantation of decellularized muscles supports muscle regeneration in volumetric muscle loss models, including nervous system and neuromuscular junctional homing. Since the nervous system plays pivotal roles during skeletal muscle regeneration and in tissue homeostasis, support of reinnervation is a crucial aspect to be considered. However, the effect of decellularized muscles on reinnervation and on neuronal axon growth has been poorly investigated. Here, we characterized residual protein composition of decellularized muscles by mas

SUBMITTER: Raffa P 

PROVIDER: S-EPMC7519766 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature