Intestinal microbiota regulates anti-tumor effect of disulfiram combined with Cu²⁺ in a mice model.

ABSTRACT:

Background

A growing number of studies show that intestinal microbiota affect the therapeutic effects of antineoplastic agents. Disulfiram (tetraethylthiuram disulfide, DSF) is an old alcohol-aversion drug that has been shown to be effective against various types of cancers in preclinical studies, while few studies are carried out to explore its mechanism.

Methods

A mice model of melanoma xenograft was generated and treated with antibiotics (Abx), disulfiram/copper (DSF/Cu²⁺ ), Abx + DSF/Cu²⁺ , and the tumor volume and survival curve were observed. Hematoxylin-eosin (HE) staining and western blotting (WB) were used to observe the protein changes related to cell morphology, inflammation, and apoptosis in tumor tissues. Quantitative real time polymerase c

SUBMITTER: Hu H 

PROVIDER: S-EPMC7520343 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature