Efficient Genome Editing in Multiple Salmonid Cell Lines Using Ribonucleoprotein Complexes.

ABSTRACT: Infectious and parasitic diseases have major negative economic and animal welfare impacts on aquaculture of salmonid species. Improved knowledge of the functional basis of host response and genetic resistance to these diseases is key to developing preventative and treatment options. Cell lines provide valuable models to study infectious diseases in salmonids, and genome editing using CRISPR/Cas systems provides an exciting avenue to evaluate the function of specific genes in those systems. While CRISPR/Cas editing has been successfully performed in a Chinook salmon cell line (CHSE-214), there are no reports to date of editing of cell lines derived from the most commercially relevant salmonid species Atlantic salmon and rainbow trout, which are difficult to transduce and therefore edit usin

SUBMITTER: Gratacap RL 

PROVIDER: S-EPMC7520412 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature