Ring finger protein 219 regulates inflammatory responses by stabilizing sirtuin 1.

ABSTRACT:

Background and purpose

Ring finger protein 219 (RNF219), a protein containing the C₃ HC₄ -type RING-HC motif, has been identified as a binding partner of the histone deacetylase sirtuin 1 (SIRT1). To explore the functions of RNF219, we examined its possible roles in the cellular responses to inflammation.

Experimental approach

Effects of RNF219 on SIRT1 were studied in vitro using RAW264.7 cells and in male BALB/c mice, treated with LPS or IFN-γ. Western blots, RT-PCR, co-immunoprecipitation and ubiquitination assays were used, along with LC-MS/MS analysis. In vivo, survival and serum cytokines and tissue levels of RNF219 and SIRT1 were measured.

Key results

Binding of RNF219 to SIRT1 inhibited degradation of SIRT1 by preventing its ubiquitinatio

SUBMITTER: Hwang JS 

PROVIDER: S-EPMC7520434 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature