Absent in melanoma 2 enhances anti-tumour effects of CAIX promotor controlled conditionally replicative adenovirus in renal cancer.

ABSTRACT: Conditionally replicative adenoviruses (CRAds) were promising approach for solid tumour treatment, but its oncolytic efficiency and toxicity are still not satisfactory for further clinical application. Here, we developed the CAIX promotor (CAIX^promotor )-controlled CRAd armed with a tumour suppressor absent in melanoma 2 (AIM2) to enhance its oncolytic potency. The CAIX^promotor -AIM2 adenoviruses (Ad-CAIX^promotor -AIM2) could efficiently express E1A and AIM2 in renal cancer cells. Compared with Ad-CAIX^promotor , Ad-CAIX^promotor -AIM2 significantly inhibited cell proliferation and enhanced cell apoptosis and cell killing, thus resulting in the oncolytic efficiency in 786-O cells or OSRC-2 cells. To explore the therapeutic effect, variou

SUBMITTER: Chai D 

PROVIDER: S-EPMC7521288 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature