The polymorphism of Hydra microsatellite sequences provides strain-specific signatures.

ABSTRACT: Hydra are freshwater polyps widely studied for their amazing regenerative capacity, adult stem cell populations, low senescence and value as ecotoxicological marker. Many wild-type strains of H. vulgaris have been collected worldwide and maintained effectively under laboratory conditions by asexual reproduction, while stable transgenic lines have been continuously produced since 2006. Efforts are now needed to ensure the genetic characterization of all these strains, which despite similar morphologies, show significant variability in their response to gene expression silencing procedures, pharmacological treatments or environmental conditions. Here, we established a rapid and reliable procedure at the single polyp level to produce via PCR amplification of three distinct microsatellite sequ

SUBMITTER: Schenkelaars Q 

PROVIDER: S-EPMC7521734 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature