Centrifugation-Assisted Immiscible Fluid Filtration for Dual-Bioanalyte Extraction.

ABSTRACT: The extraction of bioanalytes is the first step in many diagnostic and analytical assays. However, most bioanalyte extraction methods require extensive dilution-based washing processes that are not only time-consuming and laborious but can also result in significant sample loss, limiting their applications in rare sample analyses. Here, we present a method that enables the efficient extraction of multiple different bioanalytes from rare samples (down to 10 cells) without washing-centrifugation-assisted immiscible fluid filtration (CIFF). CIFF utilizes centrifugal force to drive the movement of analyte-bound glass microbeads from an aqueous sample into an immiscible hydrophobic solution to perform an efficient, simple, and nondilutive extraction. The method can be performed using convention

SUBMITTER: Juang DS 

PROVIDER: S-EPMC7521759 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature