Antibiotic resistance and genotyping of mecA-positive methicillin-resistant Staphylococcus aureus (MRSA) from milk and nasal carriage of dairy water buffaloes (Bubalus bubalis) in the Philippines.

ABSTRACT:

Objective

Mastitis is considered as an economically important disease of dairy buffaloes in Asia. This study examined the mastitis milk and nasal swab samples for the detection and genotyping of methicillin-resistant Staphylococcus aureus (MRSA) in water buffaloes.

Materials and methods

Staphylococcus aureus was identified based on biochemical tests and Polymerase Chain Reaction (PCR) detection of nuc gene, whereas MRSA on mecA gene. The disc diffusion test was used to determine the antibiotic resistance and staphylococcal cassette chromosome mec (SCCmec), spa, and multilocus sequence typing for the genotyping of isolates.

Results

Staphylococcus aureus was detected on 39/93 milk (41.94%) and 27/384 nasal swab (7.03%) sa

SUBMITTER: Badua AT 

PROVIDER: S-EPMC7521815 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature