Restoration of functional sperm production in irradiated pubertal rhesus monkeys by spermatogonial stem cell transplantation.

ABSTRACT:

Background

In male pre-pubertal cancer patients, radiation and chemotherapy impact future fertility by eradication of spermatogonial stem cells (SSCs). In macaques, spermatogenesis could be regenerated by intratesticular transplantation of SSCs, but only a small percentage of spermatozoa produced were of donor origin. Transient hormone suppression with a GnRH antagonist (GnRH-ant) enhanced spermatogenic recovery from transplanted SSCs.

Objectives

To evaluate donor-derived and endogenous spermatogenic recovery after SSC transplantation into irradiated monkeys and to test whether hormone suppression around the time of transplantation facilitates spermatogenic recovery.

Materials and methods

Testes of 15 adult rhesus monkeys were irradiated with 7 Gy and 4 months later t

SUBMITTER: Shetty G 

PROVIDER: S-EPMC7521830 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature