Characterization of a New Transposon, Tn6696, on a bla _{NDM- 1}-Carrying Plasmid From Multidrug-Resistant Enterobacter cloacae ssp. dissolvens in China.

ABSTRACT:

Background

Enterobacter cloacae is an opportunistic pathogen which is responsible for serious nosocomial infections. A gene which plays an important role in resistance to carbapenems is the New Delhi metallo-β-lactamase 1 (NDM-1). Currently, the spread of NDM-1-producing E. cloacae strains is a serious public threat.

Methods

A multidrug-resistant E. cloacae ssp. dissolvens strain CBG15936 was recovered in 2017 in Guangzhou, China. PCR, S1-pulsed-field gel electrophoresis, and Southern blotting were performed to locate the bla _{NDM - 1} gene. Susceptibility testing and conjugation experiments were also performed. Illumina HiSeq and Nanopore sequencers were used to perform whole-genome sequencing.

Results

SUBMITTER: Chen Q 

PROVIDER: S-EPMC7522282 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature