Transcriptomic and Phenotypic Analysis of a spoIIE Mutant in Clostridium beijerinckii.

ABSTRACT: SpoIIE is a phosphatase involved in the activation of the first sigma factor of the forespore, σ ^F , during sporulation. A ΔspoIIE mutant of Clostridium beijerinckii NCIMB 8052, previously generated by CRISPR-Cas9, did not sporulate but still produced granulose and solvents. Microscopy analysis also showed that the cells of the ΔspoIIE mutant are elongated with the presence of multiple septa. This observation suggests that in C. beijerinckii, SpoIIE is necessary for the completion of the sporulation process, as seen in Bacillus and Clostridium acetobutylicum. Moreover, when grown in reactors, the spoIIE mutant produced higher levels of solvents than the wild type strain. The impact of the spoIIE inactivation on gene

SUBMITTER: Diallo M 

PROVIDER: S-EPMC7522474 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature