Characterization of local SARS-CoV-2 isolates and pathogenicity in IFNAR^-/- mice.

ABSTRACT: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) recently a global pandemic with unprecedented public health, economic and social impact. The development of effective mitigation strategies, therapeutics and vaccines relies on detailed genomic and biological characterization of the regional viruses. This study was carried out to isolate SARS-CoV-2 viruses circulating in Anatolia, and to investigate virus propagation in frequently-used cells and experimental animals. We obtained two SARS-CoV-2 viruses from nasopharngeal swabs of confirmed cases in Vero E6 cells, visualized the virions using atomic force and scanning electron microscopy and determined size distribution of the particles. Viral cytopathic effects on Vero E6 cells were initially observed at 72 h post-inoculation

SUBMITTER: Hanifehnezhad A 

PROVIDER: S-EPMC7522675 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature