Isolation and characterization of Clostridium perfringens strains isolated from ostriches (Struthio camelus) in Vietnam.

ABSTRACT:

Background and aim

Clostridium perfringens can cause enteritis in ostriches. The toxin release is believed to play a major role in determining pathogenesis properties of these pathogenic bacteria. This study was conducted to isolate and characterize C. perfringens strains from ostriches in Vietnam for identifying if particular virulence factors of these pathogenic bacteria are associated with enteritis progress in ostriches.

Materials and methods

The prevalence of cpa, cpb, iA, etx, cpe, and cpb2 genes among C. perfringens isolates was determined by a multiplex polymerase chain reaction (PCR) method. The NetB toxin-encoding gene was detected by PCR and then sequenced to observe their variation. The expression of NetB toxin was checked by SD

SUBMITTER: Nguyen TT 

PROVIDER: S-EPMC7522951 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature