Optical mapping of human embryonic stem cell-derived cardiomyocyte graft electrical activity in injured hearts.

ABSTRACT:

Background

Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) show tremendous promise for cardiac regeneration, but the successful development of hESC-CM-based therapies requires improved tools to investigate their electrical behavior in recipient hearts. While optical voltage mapping is a powerful technique for studying myocardial electrical activity ex vivo, we have previously shown that intra-cardiac hESC-CM grafts are not labeled by conventional voltage-sensitive fluorescent dyes. We hypothesized that the water-soluble voltage-sensitive dye di-2-ANEPEQ would label engrafted hESC-CMs and thereby facilitate characterization of graft electrical function and integration.

Methods

We developed and validated a novel optical voltage mapping strategy based on the simulta

SUBMITTER: Filice D 

PROVIDER: S-EPMC7523067 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature