Status of kinases in Epstein-Barr virus and Helicobacter pylori Coinfection in gastric Cancer cells.

ABSTRACT:

Background

Helicobacter pylori (H. pylori) and Epstein - Barr virus (EBV) plays a significant role in aggressive gastric cancer (GC). The investigation of genes associated with these pathogens and host kinases may be essential to understand the early and dynamic progression of GC.

Aim

The study aimed to demonstrate the coinfection of EBV and H. pylori in the AGS cells through morphological changes, expression of the kinase and the probable apoptotic pathways.

Methods

Genomic DNA isolation of H. pylori and its characterization from clinical samples were performed. RT-qPCR of kinases was applied to scrutinize the gene expression of kinases in co-infected GC in a direct and indirect (separated through insert size 0.45 μm) H. pylori infection set up. Morphological changes

SUBMITTER: Sonkar C 

PROVIDER: S-EPMC7523314 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature