NormiRazor: tool applying GPU-accelerated computing for determination of internal references in microRNA transcription studies.

ABSTRACT:

Background

Multi-gene expression assays are an attractive tool in revealing complex regulatory mechanisms in living organisms. Normalization is an indispensable step of data analysis in all those studies, since it removes unwanted, non-biological variability from data. In targeted qPCR assays it is typically performed with respect to prespecified reference genes, but the lack of robust strategy of their selection is reported in literature, especially in studies concerning circulating microRNAs (miRNA). Unfortunately, this problem impedes translation of scientific discoveries on miRNA biomarkers into widely available laboratory assays. Previous studies concluded that averaged expressions of multi-miRNA combinations are more stable references than single genes. However, due to the nu

SUBMITTER: Grabia S 

PROVIDER: S-EPMC7523363 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature