Pilocytic astrocytoma demethylation and transcriptional landscapes link bZIP transcription factors to immune response.

ABSTRACT:

Background

Pilocytic astrocytoma (PA) is the most common pediatric brain tumor. While genome and transcriptome landscapes are well studied, data of the complete methylome, tumor cell composition, and immune infiltration are scarce.

Methods

We generated whole genome bisulfite sequence (WGBS) data of 9 PAs and 16 control samples and integrated available 154 PA and 57 control methylation array data. RNA sequence data of 49 PAs and 11 control samples as well as gene expression arrays of 248 PAs and 28 controls were used to assess transcriptional activity.

Results

DNA-methylation patterns of partially methylated domains suggested high stability of the methylomes during tumorigenesis. Comparing tumor and control tissues of infra- and supratentorial location using methylatio

SUBMITTER: Aichmuller CF 

PROVIDER: S-EPMC7523467 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature