Rapid detection of Mycobacterium tuberculosis in children using blood and urine specimens.

ABSTRACT:

Introduction

Laboratory and clinical features of childhood tuberculosis (TB) are non-specific and establishing an accurate diagnosis remains a challenge. This study evaluated a Single tube nested-PCR (STNPCR) to detect genomic DNA of Mycobacterium tuberculosis complex in blood and urine.

Methods

Biological samples were obtained from children (<15 years old) with clinical suspicion of pulmonary and extrapulmonary TB at public hospitals in Recife-Pernambuco, Brazil. Cultures yielded negative results in a majority of childhood TB cases, which are generally paucibacillary. A set of clinical, epidemiological, radiological, and laboratory criteria with evident clinical improvement after anti-TB treatment were frequently used to define childhood TB cases.

Results

Ninety chil

SUBMITTER: Costa-Lima JFD 

PROVIDER: S-EPMC7523525 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature