3D flow field estimation and assessment for live cell fluorescence microscopy.

ABSTRACT:

Motivation

The revolution in light sheet microscopy enables the concurrent observation of thousands of dynamic processes, from single molecules to cellular organelles, with high spatiotemporal resolution. However, challenges in the interpretation of multidimensional data requires the fully automatic measurement of those motions to link local processes to cellular functions. This includes the design and the implementation of image processing pipelines able to deal with diverse motion types, and 3D visualization tools adapted to the human visual system.

Results

Here, we describe a new method for 3D motion estimation that addresses the aforementioned issues. We integrate 3D matching and variational approach to handle a diverse range of motion without any prior on the shape of m

SUBMITTER: Manandhar S 

PROVIDER: S-EPMC7523654 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature