Protocol for parallel proteomic and metabolomic analysis of mouse intervertebral disc tissues.

ABSTRACT: The comprehensiveness of data collected by "omics" modalities has demonstrated the ability to drastically transform our understanding of the molecular mechanisms of chronic, complex diseases such as musculoskeletal pathologies, how biomarkers are identified, and how therapeutic targets are developed. Standardization of protocols will enable comparisons between findings reported by multiple research groups and move the application of these technologies forward. Herein, we describe a protocol for parallel proteomic and metabolomic analysis of mouse intervertebral disc (IVD) tissues, building from the combined expertise of our collaborative team. This protocol covers dissection of murine IVD tissues, sample isolation, and data analysis for both proteomics and metabolomics applications. The pr

SUBMITTER: Veras MA 

PROVIDER: S-EPMC7524214 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature