Functional analysis and cryo-electron microscopy of Campylobacterjejuni serine protease HtrA.

ABSTRACT: Campylobacter jejuni is a predominant zoonotic pathogen causing gastroenteritis and other diseases in humans. An important bacterial virulence factor is the secreted serine protease HtrA (HtrA _Cj ), which targets tight and adherens junctional proteins in the gut epithelium. Here we have investigated the function and structure of HtrA _Cj using biochemical assays and cryo-electron microscopy. Mass spectrometry analysis identified differences and similarities in the cleavage site specificity for HtrA _Cj by comparison to the HtrA counterparts from Helicobacter pylori and Escherichia coli. We defined the architecture of HtrA _Cj at 5.8 Å resolution as a dodecamer, built of four trimers. The contacts betw

SUBMITTER: Zarzecka U 

PROVIDER: S-EPMC7524362 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature