MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance.

ABSTRACT:

Purpose

The present study established microRNA (miRNA) expression profiles for rat ovaries displaying polycystic ovary syndrome (PCOS) with insulin resistance and explored the underlying biological functions of differentially expressed miRNAs.

Methods

A PCOS with insulin resistance rat model was created by administering letrozole and a high-fat diet. Total RNA was extracted from the ovaries of PCOS with insulin resistance rats and normal rats. Three ovaries from each group were used to identify differentially expressed miRNAs by deep sequencing. A hierarchical clustering heatmap and volcano plot were used to display the pattern of differentially expressed miRNAs. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were conducted to

SUBMITTER: Zhang C 

PROVIDER: S-EPMC7524818 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature