Lung function of primary cooks using LPG or biomass and the effect of particulate matter on airway epithelial barrier integrity.

ABSTRACT:

Background

Cooks exposed to biomass fuel experience increased risk of respiratory disease and mortality. We sought to characterize lung function and environmental exposures of primary cooking women using two fuel-types in southeastern India, as well as to investigate the effect of particulate matter (PM) from kitchens on human airway epithelial (HAE) cells in vitro.

Methods

We assessed pre- and post-bronchodilator lung function on 25 primary female cooks using wood biomass or liquified petroleum gas (LPG), and quantified exposures from 34 kitchens (PM_2.5, PM < 40 μm, black carbon, endotoxin, and PM metal and bacterial content). We then challenged HAE cells with PM, assessing its cytotoxicity to small-airway cells (A549) and its effect on: transepithelial conductan

SUBMITTER: Stapleton EM 

PROVIDER: S-EPMC7525042 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature