Recent advances in the mass spectrometric analysis of glycosphingolipidome - A review.

ABSTRACT: Aberrant expression of glycosphingolipids has been implicated in a myriad of diseases, but our understanding of the strucural diversity, spatial distribution, and biological function of this class of biomolecules remains limited. These challenges partly stem from a lack of sensitive tools that can detect, identify, and quantify glycosphingolipids at the molecular level. Mass spectrometry has emerged as a powerful tool poised to address most of these challenges. Here, we review the recent developments in analytical glycosphingolipidomics with an emphasis on sample preparation, mass spectrometry and tandem mass spectrometry-based structural characterization, label-free and labeling-based quantification. We also discuss the nomenclature of glycosphingolipids, and emerging technologies like io

SUBMITTER: Barrientos RC 

PROVIDER: S-EPMC7525043 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature