Casein kinase 1.2 over expression restores stress resistance to Leishmania donovani HSP23 null mutants.

ABSTRACT: Leishmania donovani is a trypanosomatidic parasite and causes the lethal kala-azar fever, a neglected tropical disease. The Trypanosomatida are devoid of transcriptional gene regulation and rely on gene copy number variations and translational control for their adaption to changing conditions. To survive at mammalian tissue temperatures, L. donovani relies on the small heat shock protein HSP23, the loss of which renders the parasites stress sensitive and impairs their proliferation. Here, we analysed a spontaneous escape mutant with wild type-like in vitro growth. Further selection of this escape strains resulted in a complete reversion of the phenotype. Whole genome sequencing revealed a correlation between stress tolerance and the massive amplification of a six-gene cluster on chromosome

SUBMITTER: Krober-Boncardo C 

PROVIDER: S-EPMC7525241 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature