Mitochondrial Calcium Uniporter (MCU) deficiency reveals an alternate path for Ca²⁺ uptake in photoreceptor mitochondria.

ABSTRACT: Rods and cones use intracellular Ca²⁺ to regulate many functions, including phototransduction and neurotransmission. The Mitochondrial Calcium Uniporter (MCU) complex is thought to be the primary pathway for Ca²⁺ entry into mitochondria in eukaryotes. We investigate the hypothesis that mitochondrial Ca²⁺ uptake via MCU influences phototransduction and energy metabolism in photoreceptors using a mcu^-/- zebrafish and a rod photoreceptor-specific Mcu^-/- mouse. Using genetically encoded Ca²⁺ sensors to directly examine Ca²⁺ uptake in zebrafish cone mitochondria, we found that loss of MCU reduces but does not eliminate mitochondrial Ca²⁺ uptake. Loss of MCU does not lead to photoreceptor degeneration, mildly

SUBMITTER: Bisbach CM 

PROVIDER: S-EPMC7525533 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature