Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.

ABSTRACT: Reactive oxygen species (ROS)-induced oxidative stress has been associated with diseases such as amyotrophic lateral sclerosis, stroke, and cancer. While the effect of ROS on mitochondria and endoplasmic reticulum (ER) has been well documented, its consequence on the Golgi apparatus is less well understood. In this study, we characterized the Golgi structure and function in HeLa cells after exposure to hydrogen peroxide (H₂O₂), a reagent commonly used to introduce ROS to cells. Treatment of cells with 1 mM H₂O₂ for 10 min resulted in the degradation of Arl1 and dissociation of GRIP domain-containing proteins Golgin-97 and Golgin-245 from the trans-Golgi. This effect could be rescued by treatment of cells with a ROS scavenger N-acety

SUBMITTER: Ireland SC 

PROVIDER: S-EPMC7525819 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature