Comparing methods for controlled capture and quantification of pollen in Cannabis sativa.

ABSTRACT:

Premise

Precise pollen collection methods are necessary for crop breeding, but anemophilous pollen is notoriously difficult to capture and control. Here we compared a variety of methods for the controlled capture of cannabis pollen, intended to ease the process of cross-fertilization for breeding this wind-pollinated plant, and measured the utility of light spectroscopy for quantifying relative pollen yield.

Methods and results

In two independent trials, we compared a control method of pollen collection (hand collection) to either vacuum-, water-, or bag-collection methods. We used visible light spectroscopy to quantify relative pollen yield, and validated this approach using microscopic pollen counts. We determined that pollen yield was highest when using hand collection or

SUBMITTER: Wizenberg SB 

PROVIDER: S-EPMC7526430 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature