Total Matrix Ca²⁺ Modulates Ca²⁺ Efflux via the Ca²⁺/H⁺ Exchanger in Cardiac Mitochondria.

ABSTRACT: Mitochondrial Ca²⁺ handling is accomplished by balancing Ca²⁺ uptake, primarily via the Ru360-sensitive mitochondrial calcium uniporter (MCU), Ca²⁺ buffering in the matrix and Ca²⁺ efflux mainly via Ca²⁺ ion exchangers, such as the Na⁺/Ca²⁺ exchanger (NCLX) and the Ca²⁺/H⁺ exchanger (CHE). The mechanism of CHE in cardiac mitochondria is not well-understood and its contribution to matrix Ca²⁺ regulation is thought to be negligible, despite higher expression of the putative CHE protein, LETM1, compared to hepatic mitochondria. In this study, Ca²⁺ efflux via the CHE was investigated in isolated rat cardiac mitochondria and permeabilized H9c2 cells. Mitochondria were exposed

SUBMITTER: Natarajan GK 

PROVIDER: S-EPMC7526510 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature