Human enteroviral infection impairs autophagy in clonal INS(832/13) cells and human pancreatic islet cells.

ABSTRACT:

Aim/hypothesis

Human enteroviral infections are suggested to be associated with type 1 diabetes. However, the mechanism by which enteroviruses can trigger disease remains unknown. The present study aims to investigate the impact of enterovirus on autophagy, a cellular process that regulates beta cell homeostasis, using the clonal beta cell line INS(832/13) and human islet cells as in vitro models.

Methods

INS(832/13) cells and human islet cells were infected with a strain of echovirus 16 (E16), originally isolated from the stool of a child who developed type 1 diabetes-associated autoantibodies. Virus production and release was determined by 50% cell culture infectious dose (CCID₅₀) assay and FACS analysis. The occurrence of autophagy, autophagosomes, lysosomes an

SUBMITTER: Wernersson A 

PROVIDER: S-EPMC7527364 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature