A Practical Protocol for a Comprehensive Evaluation of Sulfur Fumigation of Trichosanthis Radix Based on Both Non-Targeted and Widely Targeted Metabolomics.

ABSTRACT: Trichosanthis Radix (TR) is one of the most severely sulfur-fumigated herbs in the market, whose transformation mechanism of chemical compositions and sulfur-fumigation markers of TR have not been clarified. To excavate characteristic sulfur-fumigation markers of TR samples, this study brings up a practical protocol using both ultra-performance liquid chromatography/quadrupole time-of-flight-mass spectrum (UPLC-ESI-QTOF-MS/MS)-based non-targeted metabolomics and ultra-performance liquid chromatography/electrospray ionization/quadrupole multiple-stage linear ion-trap mass spectrum (UPLC-ESI-QTRAP-MS/MS)-based widely targeted metabolomics. The results of study demonstrated that five characteristic markers are sulfur-containing components, which were identified as p-Hydroxybenzyl hydro

SUBMITTER: Kang C 

PROVIDER: S-EPMC7527402 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature