Toward diagnostic relevance of the α_Vβ₅, α_Vβ₃, and α_Vβ₆ integrins in OA: expression within human cartilage and spinal osteophytes.

ABSTRACT: We previously reported ¹⁸FPRGD₂ uptake by the coxofemoral lining, intervertebral discs and facet joint osteophytes in OA using PET/SCAN imaging. However, the molecular mechanism by which the PRGD₂ tracer interacts with joint tissues and osteophytes in OA remains unclear. As PRGD₂ ligands are expected to belong to the RGD-specific integrin family, the purpose of this study was (i) to determine which integrin complexes display the highest affinity for PRGD2-based ligands, (ii) to analyze integrin expression in relevant tissues, and (iii) to test integrin regulation in chondrocytes using OA-related stimuli to increase the levels of fibrosis and ossification markers. To this end, the affinity of PRGD₂-based ligands for five heterodimeric i

SUBMITTER: Charlier E 

PROVIDER: S-EPMC7527564 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature