Implementation of Microfluidics for Antimicrobial Susceptibility Assays: Issues and Optimization Requirements.

ABSTRACT: Despite the continuous emergence of multi-drug resistant pathogens, the number of new antimicrobials reaching the market is critically low. Natural product peptides are a rich source of bioactive compounds, and advances in mass spectrometry have achieved unprecedented capabilities for the discovery and characterization of novel molecular species. However, traditional bioactivity assay formats hinder the discovery and biochemical characterization of natural product antimicrobial peptides (AMPs), necessitating large sample quantities and significant optimization of experimental parameters to achieve accurate/consistent activity measurements. Microfluidic devices offer a promising alternative to bulk assay systems. Herein, a microfluidics-based bioassay was compared to the traditional 96-well

SUBMITTER: Parsley NC 

PROVIDER: S-EPMC7527609 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature