Next-generation sequencing in the diagnosis of viral encephalitis: sensitivity and clinical limitations.

ABSTRACT: Identification of pathogens causing viral encephalitis remains challenging, and in over 50% of cases the etiologic factor remains undetermined. Next-generation sequencing (NGS) based metagenomics has been successfully used to detect novel and rare infections, but its value for routine diagnosis of encephalitis remains unclear. The aim of the present study was to determine the sensitivity of shotgun metagenomic sequencing protocols, which include preamplification, and testing it against cerebrospinal fluid (CSF) samples from encephalitis patients. For sensitivity testing HIV and HBV positive sera were serially diluted in CSF from an uninfected patient. NGS repeatedly detected HIV and HBV sequences present at concentrations from 10⁵ to 10² and from 10⁵ to 10

SUBMITTER: Perlejewski K 

PROVIDER: S-EPMC7528011 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature