TCR sequencing paired with massively parallel 3' RNA-seq reveals clonotypic T cell signatures.

ABSTRACT: High-throughput 3' single-cell RNA-sequencing (scRNA-seq) allows cost-effective, detailed characterization of individual immune cells from tissues. Current techniques, however, are limited in their ability to elucidate essential immune cell features, including variable sequences of T cell antigen receptors (TCRs) that confer antigen specificity. Here, we present a strategy that enables simultaneous analysis of TCR sequences and corresponding full transcriptomes from 3'-barcoded scRNA-seq samples. This approach is compatible with common 3' scRNA-seq methods, and adaptable to processed samples post hoc. We applied the technique to identify transcriptional signatures associated with T cells sharing common TCRs from immunized mice and from patients with food allergy. We observed preferential p

SUBMITTER: Tu AA 

PROVIDER: S-EPMC7528220 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature