Altering the Solubility of the Antibiotic Candidate Nisin-A Computational Study.

ABSTRACT: The growing bacterial resistance to available antibiotics makes it necessary to look for new drug candidates. An example is the lanthionine-containing nisin, which has a broad spectrum of antimicrobial activity. While nisin is widely utilized as a food preservative, its poor solubility and low stability at physiological pH hinder its use as an antibiotic. As the solubility of nisin is controlled by the residues of the hinge region, we have performed molecular dynamics simulations of various mutants and studied their effects on nisin's solubility. These simulations are complicated by the presence of two uncommon residues (dehydroalanine and dehydrobutyrine) in the peptide. The primary goal of the present study is to derive rules for designing new mutants that will be more soluble at physiological pH and, therefore, may serve as a basis for the future antibiotic design. Another aim of our study is to evaluate whether existing force fields can model the solubility of these amino acids accurately in order to motivate further developments of force fields to account for solubility information.

SUBMITTER: Pandey P 

PROVIDER: S-EPMC7528279 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature