Bias of marker genes in PCR of anammox bacteria in natural habitats.

ABSTRACT: The identification of anammox bacteria is mostly relied on PCR with various marker genes. However, the community composition revealed by different marker genes and whether the marker genes influence the resulted community composition remain unclear. We compared the community structure of anammox bacteria in enriched and natural environments revealed by 16S rRNA and functional genes (hzo, hzsA and hzsB) from public database and published papers. The genus of Ca. Scalindua showed the lowest similarities with other genera, especially for the hzsA gene (66.9%-68.6%). The 16S rRNA gene is the most commonly used marker gene in natural habitats with 151 out 221 papers in total. The anammox bacterial community composition is distributed according to the source of habitat regardless the use of vari

SUBMITTER: Cai M 

PROVIDER: S-EPMC7529225 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature