Quantitative multiplex immunofluorescence analysis identifies infiltrating PD1⁺ CD8⁺ and CD8⁺ T cells as predictive of response to neoadjuvant chemotherapy in breast cancer.

ABSTRACT:

Background

This study aimed to explore the potentially predictive role and dynamic changes of immune checkpoints on T cell subsets in patients with breast cancer receiving neoadjuvant chemotherapies.

Methods

Fluorescent multiplex immunohistochemistry (mIHC) was used to stain CD4, CD8, PD1, TIM3, and cytokeratins simultaneously in paired breast cancer samples before and after neoadjuvant therapies (NAT) in a prospective cohort (n = 50). Singleplex IHC was conducted to stain for CD3 in 100 cases with inclusion of extra retrospective 50 cases. Cell levels were correlated with clinicopathological parameters and pathological complete response (pCR).

Results

In pretreatment tumors, the percentages of infiltrating CD8⁺ , PD1⁺ , PD1⁺ CD8⁺

SUBMITTER: Liang H 

PROVIDER: S-EPMC7529566 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature