Polystyrene adsorbents: rapid and efficient surrogate for dialysis in membrane protein purification.

ABSTRACT: Membrane protein purification is a laborious, expensive, and protracted process involving detergents for its extraction. Purifying functionally active form of membrane protein in sufficient quantity is a major bottleneck in establishing its structure and understanding the functional mechanism. Although overexpression of the membrane proteins has been achieved by recombinant DNA technology, a majority of the protein remains insoluble as inclusion bodies, which is extracted by detergents. Detergent removal is essential for retaining protein structure, function, and subsequent purification techniques. In this study, we have proposed a new approach for detergent removal from the solubilized extract of a recombinant membrane protein: human phospholipid scramblase 3 (hPLSCR3). N-lauryl sarcosine

SUBMITTER: Palanirajan SK 

PROVIDER: S-EPMC7529760 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature