An on-chip wound healing assay fabricated by xurography for evaluation of dermal fibroblast cell migration and wound closure.

ABSTRACT: Dermal fibroblast cell migration is a key process in a physiological wound healing. Therefore, the analysis of cell migration is crucial for wound healing research. In this study, lab-on-a-chip technology was used to investigate the effects of basic fibroblast growth factor (bFGF), mitomycin C (MMC), MEK1/2 inhibitor (U0126) and fetal calf serum (FCS) on human dermal fibroblast cell migration. The microdevice was fabricated consisting of microchannels, pneumatic lines and pneumatically-activated actuators by xurographic rapid prototyping. In contrast to current approaches in in vitro wound healing such as scratch assays and silicone inserts in wellplate format, which show high variability and poor reproducibility, the current system aims to automate the wounding procedure at high precision

SUBMITTER: Shabestani Monfared G 

PROVIDER: S-EPMC7529912 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature