Placental Mitochondria Therapy for Cerebral Ischemia-Reperfusion Injury in Mice.

ABSTRACT:

Background and purpose

There is an urgent need to develop adjunct therapies that can be added onto reperfusion for acute ischemic stroke. Recently, mitochondrial transplantation has emerged as a promising therapeutic approach for boosting brain tissue protection. In this proof-of-concept study, we investigate the feasibility of using placenta as a source for mitochondrial transplantation in a mouse model of transient focal cerebral ischemia-reperfusion.

Methods

Mitochondria-enriched fractions were isolated from cryopreserved mouse placenta. Mitochondrial purity and JC1 membrane potentials were assessed by flow cytometry. Adenosine triphosphate and mitochondrial proteins were measured by luminescence intensity and western blot, respectively. Therapeutic efficacy of mitochondr

SUBMITTER: Nakamura Y 

PROVIDER: S-EPMC7530055 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature