Sigma-1 receptor ablation impedes adipocyte-like differentiation of mouse embryonic fibroblasts.

ABSTRACT: The sigma-1 receptor (Sig1R) is a unique ligand-operated endoplasmic reticulum (ER) protein without any mammalian homolog. It has long been a pharmacological target for intervention of psychiatric disorders, and recently garnered refreshed interest for its neuroprotective potential. Though reported to modulate various intracellular events, its influence on cell identity is little known. We explored a role for Sig1R in adipocyte differentiation. We induced adipogenic differentiation of mouse embryonic fibroblasts (MEFs) with a differentiation medium. MEFs were isolated from Sigmar1^-/- and Sigmar1^+/+ mice. The induced adipocyte-like phenotype was detected through Western blots of master transcription factors (PPARγ, CEBPA, SREBP1, SREBP2), lipogenic proteins (FABP4, ACC

SUBMITTER: Yang H 

PROVIDER: S-EPMC7530065 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature