Collateral Toxicity Limits the Evolution of Bacterial Release Factor 2 toward Total Omnipotence.

ABSTRACT: When new genes evolve through modification of existing genes, there are often tradeoffs between the new and original functions, making gene duplication and amplification necessary to buffer deleterious effects on the original function. We have used experimental evolution of a bacterial strain lacking peptide release factor 1 (RF1) in order to study how peptide release factor 2 (RF2) evolves to compensate the loss of RF1. As expected, amplification of the RF2-encoding gene prfB to high copy number was a rapid initial response, followed by the appearance of mutations in RF2 and other components of the translation machinery. Characterization of the evolved RF2 variants by their effects on bacterial growth rate, reporter gene expression, and in vitro translation termination reveals a complex p

SUBMITTER: Abdalaal H 

PROVIDER: S-EPMC7530605 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature