FUBP1 and FUBP2 enforce distinct epigenetic setpoints for MYC expression in primary single murine cells.

ABSTRACT: Physiologically, MYC levels must be precisely set to faithfully amplify the transcriptome, but in cancer MYC is quantitatively misregulated. Here, we study the variation of MYC amongst single primary cells (B-cells and murine embryonic fibroblasts, MEFs) for the repercussions of variable cellular MYC-levels and setpoints. Because FUBPs have been proposed to be molecular "cruise controls" that constrain MYC expression, their role in determining basal or activated MYC-levels was also examined. Growing cells remember low and high-MYC setpoints through multiple cell divisions and are limited by the same expression ceiling even after modest MYC-activation. High MYC MEFs are enriched for mRNAs regulating inflammation and immunity. After strong stimulation, many cells break through the ceiling an

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC7530719 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature