A robust multiplex immunofluorescence and digital pathology workflow for the characterisation of the tumour immune microenvironment.

ABSTRACT: Multiplex immunofluorescence is a powerful tool for the simultaneous detection of tissue-based biomarkers, revolutionising traditional immunohistochemistry. The Opal methodology allows up to eight biomarkers to be measured concomitantly without cross-reactivity, permitting identification of different cell populations within the tumour microenvironment. In this study, we aimed to validate a multiplex immunofluorescence workflow in two complementary multiplex panels and evaluate the tumour immune microenvironment in colorectal cancer (CRC) formalin-fixed paraffin-embedded tissue. We stained CRC and tonsil samples using Opal multiplex immunofluorescence on a Leica BOND RX immunostainer. We then acquired images on an Akoya Vectra Polaris and performed multispectral unmixing using inform. Antib

SUBMITTER: Viratham Pulsawatdi A 

PROVIDER: S-EPMC7530793 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature