Reconstruction and analysis of genome-scale metabolic model of weak Crabtree positive yeast Lachancea kluyveri.

ABSTRACT: Lachancea kluyveri, a weak Crabtree positive yeast, has been extensively studied for its unique URC pyrimidine catabolism pathway. It produces more biomass than Saccharomyces cerevisiae due to the underlying weak Crabtree effect and resorts to fermentation only in oxygen limiting conditions that renders it as a suitable industrial host. The yeast also produces ethyl acetate as a major overflow metabolite in aerobic conditions. Here, we report the first genome-scale metabolic model, iPN730, of L. kluyveri comprising of 1235 reactions, 1179 metabolites, and 730 genes distributed in 8 compartments. The in silico viability in different media conditions and the growth characteristics in various carbon sources show good agreement with experimental data. Dynamic flux balance analysis describes th

SUBMITTER: Nanda P 

PROVIDER: S-EPMC7530994 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature