Chromatin arranges in chains of mesoscale domains with nanoscale functional topography independent of cohesin.

ABSTRACT: Three-dimensional (3D) chromatin organization plays a key role in regulating mammalian genome function; however, many of its physical features at the single-cell level remain underexplored. Here, we use live- and fixed-cell 3D super-resolution and scanning electron microscopy to analyze structural and functional nuclear organization in somatic cells. We identify chains of interlinked ~200- to 300-nm-wide chromatin domains (CDs) composed of aggregated nucleosomes that can overlap with individual topologically associating domains and are distinct from a surrounding RNA-populated interchromatin compartment. High-content mapping uncovers confinement of cohesin and active histone modifications to surfaces and enrichment of repressive modifications toward the core of CDs in both hetero- and euch

SUBMITTER: Miron E 

PROVIDER: S-EPMC7531892 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature